Immunohistochemistry (IHC) is a powerful tool that allows investigators to study protein expression in tissue samples with a spatial context. It serves diagnostic, prognostic and predictive roles in a variety of conditions and is also widely used in basic science and clinical research.

The ihc histology service of IHC relies on many factors. For example, samples must be properly fixed to preserve tissue structure. This process is complex and requires precise measurement of the time, temperature, and pH of fixatives. Uneven or poorly-adhering sections produce variable results and may obscure the presence of a target antigen. Additionally, it is important to use a chromogen that can be easily distinguished from background staining and to provide appropriate internal positive and negative controls for IHC analysis.

IHC Histology Service Providers: Finding the Best Fit for Your Research

To ensure the best results, it is important to follow established protocols for handling and storing the samples. This includes proper storage in a preservative like formalin and careful labeling. In addition, IHC reagents must be stored at a specific temperature to prevent degradation and to ensure that a sufficient amount of the reagent is present for optimal staining.

The ihc histology service offers IHC services to support both basic science and clinical studies. The service provides tissue grossing/cassetting, paraffin processing, sectioning and routing, as well as routine hematoxylin and eosin and special stains. The core lab is staffed by American Society of Clinical Pathology-certified professionals. Investigators can request IHC services by submitting a iLab request. Pricing is competitive and discounts are available for PIs in the PSR Biobank.